研究论文
Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method
Kenneth J. Livak, Thomas D. Schmittgen
Washington State University
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参考文献 12
Quantitation of thymidylate synthase, dihydrofolate reductase, and DT-diaphorase gene expression in human tumors using the polymerase chain reaction.
被引 246T. Horikoshi, Kathleen D. Danenberg, Thomas Stadlbauer · PubMed · 1992
Selective Degradation of Early-Response-Gene mRNAs: Functional Analyses of Sequence Features of the AU-Rich Elements
被引 252Chyi‐Ying A. Chen, Ann‐Bin Shyu · Molecular and Cellular Biology · 1994
Development and Validation of Real-Time Quantitative Reverse Transcriptase–Polymerase Chain Reaction for Monitoring Gene Expression in Cardiac Myocytesin Vitro
被引 1,398Jane Winer, Christopher Kwang S. Jung, Irene Shackel · Analytical Biochemistry · 1999
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引用本文 184,352
Analyzing real-time PCR data by the comparative CT method
被引 27,062Thomas D. Schmittgen, Kenneth J. Livak · Nature Protocols · 2008
Normalization of Real-Time Quantitative Reverse Transcription-PCR Data: A Model-Based Variance Estimation Approach to Identify Genes Suited for Normalization, Applied to Bladder and Colon Cancer Data Sets
被引 7,252Claus L. Andersen, Jens Ledet Jensen, Torben F. Ørntoft · Cancer Research · 2004
Real-time quantification of microRNAs by stem-loop RT-PCR
被引 5,037C. Chen · Nucleic Acids Research · 2005
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