Study On Magnesium-Containing Dual-Release System For Osteogenesis

This study aims to investigate the osteogenic potential of magnesium ions (Mg²⁺) at varying concentrations to promote early-stage vascularization and new bone formation. A specialized dual-release system was designed to achieve targeted delivery and controlled release of Mg²⁺, thereby enhancing alveolar bone regeneration. magnesium-loaded microsphere-hydrogel dual-release scaffold** was fabricated. Key evaluations included: In vitro assays: Mg²⁺ release kinetics, physicochemical characterization, cell migration, cell adhesion, and immunocytochemical analysis to assess angiogenesis and osteogenic capacity. In vivo evaluation: Implantation into rabbit mandibles followed by retrieval at 4, 8, and 12 weeks for immunohistochemical analysis, Micro-CT scanning, and Real-time PCR to quantify vascularization and osteogenic marker expression (e.g., VEGF, OPN, OCN). Initial release phase: stimulated cell migration and adhesion. Sustained release phase: significantly upregulating angiogenesis-related factors (e.g., VEGF) and osteogenic markers (OPN, OCN). In vivo outcomes: Enhanced vascular network formation and progressive bone mineralization were observed The Mg²⁺ dual-release system demonstrates stage-specific release kinetics. Early stage: Accelerated angiogenesis via controlled Mg²⁺ release. Late stage: Promoted osteogenic differentiation and matrix mineralization. This system offers a promising strategy for spatiotemporal regulation of bone regeneration, particularly in dental and orthopedic applications.