PEDOT/FeOOH/BiVO₄ Nanohybrids with Excellent Photoelectric Performance Promoted by Photothermal Effects for the Ultrasensitive Detection of MicroRNA-375-3p

Herein, a novel photoactive poly(3,4-ethyl-enedioxythiophene) (PEDOT)/FeOOH/BiVO 4 nanohybrid with excellent photoelectrochemical (PEC) efficiency was assembled for the construction of an ultrasensitive biosensor for microRNA-375-3p (miRNA-375-3p) detection. In comparison with the traditional FeOOH/BiVO 4 photoactive composite, the PEDOT/FeOOH/BiVO 4 nanohybrids exhibited markedly enhanced photocurrent due to the promoted interfacial charge separation by PEDOT, which was used not only as an electron conductor but also as a localized photothermal heater to enhance the photogenerated carrier separation. Based on this PEDOT/FeOOH/BiVO 4 photoelectrode and an enzyme-free signal amplification strategy including a target-induced catalytic hairpin assembly (CHA) and hybridization chain reaction (HCR), a PEC sensing platform for the detection of miRNA-375-3p was established, achieving a wide linear range from 1 fM to 10 pM with a low detection limit of 0.3 fM. Moreover, this work provides a general photocurrent enhancement strategy for the development of high-performing PEC biosensors for sensitive detection of biomarkers and early disease diagnosis.