A Cu ₂ O/hemin-GO nanozyme with aptamer-enhanced peroxidase-mimic activity for colorimetric detection of kanamycin in milk

O/hemin-GO nanozyme and a corresponding reduction in color change. The aptasensor exhibited excellent sensitivity with a linear detection range from 0.1 pM to 1 μM and a detection limit of 16 fM. The recovery rates for KAN in milk samples ranged from 92.05% to 110.71%. Furthermore, the colorimetric aptasensor demonstrated high selectivity and reproducibility. Overall, the colorimetric approach achieved the economical, simple and sensitive detection of KAN residues, indicating its promising potential in real-world applications. In addition, this sensing method could be applied to other targets by replacing the aptamer, providing a strategy for developing sensors facilitating rapid detection.