Separation of tumor cells from bronchoalveolar lavage fluid (BALF) via microgrooves enhanced immiscible filtration assisted by surface tension (ME-IFAST)
Yongbing Chen, Jun Zhao, Yaoping Liu, Menghe Yuan, Wei Wang, Ming Chu, Yuedan Wang, Haichao Li
Peking University Institute of Microelectronics National Key Laboratory of Science and Technology on Micro/Nano Fabrication
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Separation and characterization of lung cancer cells is of great importance to the diagnosis and treatment assessment of this disease. Here, we reported a microgrooves-enhanced IFAST strategy (ME-IFAST) for tumor cell separation from clinical BALF sample. After the process, we enhanced the efficiency of beads bonded on each cell, from around 14.6 to 28.8 beads per cell, and decreased the number of un-bonded beads by 30.2% as well as cells with no bonded beads. It has been proved to be an efficient size amplification performance with a low free magnetic beads background.
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工程Microfluidic and Bio-sensing Technologies
Microfluidic and Capillary Electrophoresis Applications · Electrowetting and Microfluidic Technologies
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