Figure S12 from TENT5A Maintains MYC mRNA Stability to Enhance Osteosarcoma Stemness

Figure S12. Sensitivity of osteosarcoma cell lines to Ro-3306 treatment in vitro. Related to Figure 6b. (a) Dose-response curves showing cell viability and calculated IC50 values for multiple osteosarcoma cell lines (U2OS, SJSA, HOS, HOS/MNNG, 143B, MG63, MG63.2, SaOS-2, DUNN) treated with Ro-3306. Data represent mean ± SEM. n=6 technical replicates for each strain. (b) Colony formation assays and quantification of osteosarcoma cell line proliferation following Ro-3306 treatment at indicated concentrations (0, 5, and 10 µM). Quantifications expressed as relative grey values (mean ± SEM). Statistical significance indicated as ****p < 0.0001. n=3 technical replicates for each strain. (c) Representative images and quantification of live/dead assays using Calcein-AM (green, viable cells) and propidium iodide (PI, red, dead cells) staining in osteosarcoma cell lines treated with Ro-3306 (2μM). Scale bars:100 µm. Data shown as mean ± SEM. n=3 technical replicates for each strain