Klotho and SIRT1 changes from pre-diabetes to diabetes and pre-hypertension to hypertension

BACKGROUND: Hypertension and diabetes are among the most important risk factors of cardiovascular diseases. Klotho and SIRT1 are known as anti-aging factors with beneficial effects on cardiovascular system. In this study we investigated the serum Klotho and SIRT1 levels in pre-diabetic and pre-hypertensive individuals and then in diabetic and hypertensive patients to see their relationship with these diseases. METHOD: 229 individuals divided into six groups with similar gender and age distribution 1-Control (normal BP and FBS) 2-pre-diabetic (FBS between 100 and 125 mg/dl) 3-diabetic (FBS ≥ 126 mg/dl), 4-pre-hypertensive (SBP 120-139 or DBP 80-89 mm Hg) 5-hypertensive (SBP ≥ 140 or DBP ≥ 90 mm Hg), and 6-patients with combined hypertension/diabetes. Serum levels of Klotho and SIRT1 were measured by ELISA method. RESULTS: Serum Klotho and STRT1 levels decreased in pre-diabetes and returned to normal in diabetic patients. Their concentration increased in pre-hypertension and recovered to normal in hypertension. In the physiologic range of FBS there is a negative correlation between Klotho and SIRT1 with FBS. When pathologic ranges of FBS added to analysis, the negative correlation abolished/U shaped. Also an inverse U shape correlation observed between Klotho and SIRT1 with MAP in the range of normal to hypertensive BP levels. There was an overall positive relationship between the serum levels of Klotho and SIRT1 themselves. CONCLUSION: The serum levels of the anti-aging proteins Klotho and SIRT1 increases or reduces at the onset of the disease, as a compensatory mechanism, but as the disease progresses their level recovers.