[Mechanism study on electroacupuncture regulating IL-4/JAK1/STAT6 signaling pathway to promote M2 macrophage polarization and improve intestinal mucosal barrier injury in mice with ulcerative colitis].

OBJECTIVES: To observe the effect of electroacupuncture(EA) intervention on the interleukin-4 (IL-4)/Janus kinase 1 (JAK1)/signal transducer and activator of transcription 6 (STAT6) signaling pathway in colon tissue of chronic ulcerative colitis (UC) mouse model, in order to explore the potential mechanism of EA in promoting M2 macrophage polarization and improving intestinal mucosal barrier injury in UC mice. METHODS: ratio in mesenteric lymph nodes, and CD206 and arginase-1 (Arg-1) positive expressions in colon tissue by flow cytometry and immunofluorescence staining, respectively. RESULTS: 0.05). CONCLUSIONS: EA can promote the repair of colonic mucosal barrier in mice with chronic UC and alleviate symptoms such as diarrhea and hematochezia, which may be associated with the activation of IL-4/JAK1/STAT6 signaling pathway and the inducing of M2 macrophage polarization in colon tissue.