Studies on the Index Compounds for HPLC Analysis of Glycyrrhiza uralensis

HPLC of the AcOEt extracts of subterranean (roots or stolons)samples of Glycyrrhiza uralensis showed two types of the reproducible chromatograms distinct from those of other species ( G. glabra, G. pallidiflora, G. echinam, G. macedonica ).As index compounds for HPLC of G.uralensis, two new compounds, named glyurallins A and B, were isolated and their structures were defined, in addition to identification of nine compounds to glycycoumarin, semilicoisoflavone B, dehydroglyasperin C, licoisoflavanone, glycyrol, glycyrin, gancaonin N, licoricidine and 8y , ydimethylallylwighteone.Licorice, dried roots and stolons of Glycyrrhiza uralensis, G. glabra and some other species are an important source of traditional Chinese medicines, materials for glycyrrhizin preparation, and food and tobacco ingredients.lWe report in this paper that two new compounds, named glyurallins A and B were obtained along with nine known compounds from the AcOEt extracts of subterranean samples of G. uralensis and those of commercially available samples of Glycyrrhizae Radix.These 11 compounds were found