Genome-wide analysis of GRAS and CXE and interactions between SbDELLAs and SbGID1s in Scutellaria baicalensis Georgi

A total of 58 SbGRAS and 43 SbCXE family members were identified using phylogenetic analysis, based on the S. baicalensis genome database. And four DELLA subfamily members (SbGRAS1-4) and two gibberellin receptors (SbGID1A and SbGID1B) were identified. This study found that SbDELLAs and SbGID1s significantly responded to GA3 and PAC treatments. GA signal transduction depends on the interactions between SbDELLAs and SbGID1s, SbGRAS1 and SbGRAS2 can interact with GID1s regardless of the presence of GA3, and this interaction is accelerated when GA3 is added. This suggests that SbGRAS1 and SbGRAS2 play a more critical role in gibberellin signaling. This study provides the systematic analysis and investigation of two gene families, and offers guidance for regulating flavonoid metabolism in the gibberellin signaling pathway in S. baicalensis.